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rabbit igg anti human phosphor egfr tyr1068  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit igg anti human phosphor egfr tyr1068
    Rabbit Igg Anti Human Phosphor Egfr Tyr1068, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphor+egfr/Phospho-EGF+Receptor+(Tyr1068)+XP+Rabbit+mAb/us12454577-478-3-10
    Average 96 stars, based on 1362 article reviews
    rabbit igg anti human phosphor egfr tyr1068 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Luminescence Assay:

    Article Title: Design, synthesis and biological evaluation of aminopyrimidine derivatives bearing dihydroquinoxalinone as novel EGFRL858R/T790M kinase inhibitors against non-small-cell lung cancer
    Article Snippet: .. Materials RPMI-1640 medium (ATCC, Rockville, MD, USA, 30-2001), MEM medium (Gibco, Invitrogen Corporation, NY, USA, 11095080), penicillin and streptomycin (Sigma, St. Louis, MO, USA), L-alany-L-glutamine (Zhongqiaoxinzhou Biotech, China, CSP004), sodium pyrurate (Sigma, USA, S9133), Kinase substrate 22 (GL, China, 112393), CCK8 (Beyotime, Shanghai, China), 96- well plates (Biosahrp, BS-MP-96W), CellTiter-Glo luminescent assay (Promega-G7573, USA), Osimertinib (MedChemExpress, Shanghai, China), FITC-conjugated Annexin V (KeyGEN Biotech, NanJing, China, KGA108), PMSF (Solon, OH, USA), NC membranes (Millipore, Schwalbach, Germany), phosphor-EGFR (CST, Tyr1068, #3777T), EGFR (AB clonal, #A11351), phosphor-AKT (Protein-tech, Ser473, #66444-1-Ig), AKT (CST, #4691S) and GAPDH (Protein-tech, #60004-1-Ig). .. Cell culture H1975 (human lung adenocarcinoma cell line) and A549 (human lung adenocarcinoma cell line) (ATCC, Rockville, MD, USA) were cultured in RPMI1640 medium (ATCC, Rockville, MD, USA, 30-2001) supplemented with 10% FBS, penicillin (100 units/ml), and streptomycin (100 units/ml) (Sigma, St. Louis, MO, USA), respectively, in a humidified atmosphere with 5% CO2 at 37 °C.

    Staining:

    Article Title: Anabolic phenotype in cartilage-specific mitogen-inducible gene-6 knockout mice is independent of transforming growth factor-α
    Article Snippet: .. Sections were then stained overnight at 4 °C using primary antibody for SOX9 (R&D Systems, AF3075) or phosphor-EGFR (phosphoTyr-1173; Cell Signaling Technology), and incubated with secondary antibody conjugated to horseradish peroxidase (Santa Cruz Biotechnology). .. Sections were exposed to DAB + chromogen (Dako Canada) and counterstained with 0.5% methyl green in 0.1 M sodium acetate buffer 4.2 pH.

    Article Title: Anabolic phenotype in cartilage-specific Mitogen-inducible gene-6 knockout mice is independent of Transforming growth factor- α
    Article Snippet: .. Sections were then stained overnight at 4°C using primary antibody for SOX9 (R&D Systems) or phosphor-EGFR (phosphoTyr-1173; Cell Signaling Technology), and incubated with secondary antibody conjugated to horseradish peroxidase (Santa Cruz Biotechnology). .. Sections were exposed to DAB+ chromogen (Dako Canada) and counterstained with 0.5% methyl green in 0.1M sodium acetate buffer 4.2 pH.

    Incubation:

    Article Title: Anabolic phenotype in cartilage-specific mitogen-inducible gene-6 knockout mice is independent of transforming growth factor-α
    Article Snippet: .. Sections were then stained overnight at 4 °C using primary antibody for SOX9 (R&D Systems, AF3075) or phosphor-EGFR (phosphoTyr-1173; Cell Signaling Technology), and incubated with secondary antibody conjugated to horseradish peroxidase (Santa Cruz Biotechnology). .. Sections were exposed to DAB + chromogen (Dako Canada) and counterstained with 0.5% methyl green in 0.1 M sodium acetate buffer 4.2 pH.

    Article Title: Anabolic phenotype in cartilage-specific Mitogen-inducible gene-6 knockout mice is independent of Transforming growth factor- α
    Article Snippet: .. Sections were then stained overnight at 4°C using primary antibody for SOX9 (R&D Systems) or phosphor-EGFR (phosphoTyr-1173; Cell Signaling Technology), and incubated with secondary antibody conjugated to horseradish peroxidase (Santa Cruz Biotechnology). .. Sections were exposed to DAB+ chromogen (Dako Canada) and counterstained with 0.5% methyl green in 0.1M sodium acetate buffer 4.2 pH.

    Article Title: SLC12A8 mediates TKI resistance in EGFR-mutant lung cancer via PDK1/AKT axis.
    Article Snippet: Purpose Epidermal growth factor receptor (EGFR) mutation is a prominent driver of lung cancer.. Tyrosine kinase inhibitors (TKIs) have shown efficacy in treating EGFR-mutant lung cancer, but the emergence of drug resistance poses a significant challenge.. Recent research has highlighted solute carrier family 12 member 8 (SLC12A8) as one of the highly upregulated genes in various cancer types.

    other:

    Article Title: Irreversible tyrosine kinase inhibitors induce the endocytosis and downregulation of ErbB2
    Article Snippet: Antibodies to EGFR, phosphor-EGFR (Tyr1068), phosphor-ErbB2 (Tyr1196), total AKT, phosphor-AKT, total ERK1/2, phosphor-ERK1/2, LAMP1, and Rab7 were from Cell Signaling Technology (Danvers, MA).

    Binding Assay:

    Article Title: SLC12A8 mediates TKI resistance in EGFR-mutant lung cancer via PDK1/AKT axis.
    Article Snippet: Purpose Epidermal growth factor receptor (EGFR) mutation is a prominent driver of lung cancer.. Tyrosine kinase inhibitors (TKIs) have shown efficacy in treating EGFR-mutant lung cancer, but the emergence of drug resistance poses a significant challenge.. Recent research has highlighted solute carrier family 12 member 8 (SLC12A8) as one of the highly upregulated genes in various cancer types.



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    Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of <t>EGFR,</t> β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.
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    Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of <t>EGFR,</t> β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.
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    Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of <t>EGFR,</t> β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.
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    Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of <t>EGFR,</t> β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.
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    Image Search Results


    Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of EGFR, β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.

    Journal: Oncology Letters

    Article Title: The feasible role of soluble E‑cadherin in spheroidogenesis of HCT116 colorectal cancer cells, a candidate biomarker for liquid biopsy

    doi: 10.3892/ol.2025.14991

    Figure Lengend Snippet: Figure 4. Characteristics of long‑term‑maintained spheroids of WT and KO HCT116 cells. (A) Western blotting showing significant increase of E‑cadherin in spheroids (3D) as compared to conventional 2D culture in both cell lines. (B) ELISA on culture media showing time‑dependent increases of soluble E‑cadherin in spheroid culture, with such increases being prominent in WT HCT116 cells. (C) Western blotting showing significant increases of EGFR, β‑catenin, pan‑RAS, and ERK activation in spheroid culture as compared to 2D cultures in both cell lines. *P<0.05, **P<0.01, ***P<0.001 vs. 2D; #P<0.05, ##P<0.01, ###P<0.001 vs. WT at same time. WT, wild‑type; KO, knock‑out; 2D, 2‑dimensional; 3D, 3‑dimensional.

    Article Snippet: A, Reagents Name (cat. no.) Company Purpose/action MTT (M6494) Thermo Fisher Scientific Cell viability 5‐FU (F6627); erlotinib (S2156) Sigma‐Aldrich (Merck KGaA) Anti‐cancer drugs Saracatinib (11497) Cayman Chemical T‐PERTM protein extraction reagent (78510) Thermo Fisher Scientific Preparation for western blotting Protease inhibitor cocktail (ab201111); Abcam Preparation for western blotting phosphatase inhibitor cocktail I (ab201112); phosphatase inhibitor cocktail II (ab201113) PierceTM BCA protein assay kit (23227) Thermo Fisher Scientific Preparation for western blotting PD98059 (9900) Cell Signaling Tech MEK‐ERK inhibitor Hematoxylin (H‐3404) Vector Laboratories Counter‐staining Human E‐cadherin protein (ab235682) Abcam Recombinant E‐cadherin protein for spheroidogenesis DECMA‐1 (ab11512) Abcam Anti‐E‐cadherin antibody (intercellular junction marker) for spheroidogenesis Human E‐cadherin SimpleStep ELISA Kit (ab233611) Abcam Soluble E‐cadherin detection B, Antibodies Name (cat. no.) Company Purpose/action Phosphor‐ERK (4370) Cell Signaling Tech Primary antibody EGFR (ab52894) Abcam Primary antibody ERK (sc‐93); β‐catenin (sc‐7199); Santa Cruz Biotechnology Primary antibody pan‐RAS (sc‐166691); β‐actin (sc‐47778); GAPDH (sc‐47724) Anti‐mouse IgG (PI‐2000); anti‐rabbit IgG (PI‐1000) Vector Laboratories Secondary antibody ImmPRESS Reagent kit (MP‐7401) Vector Laboratories Immunocytochemistry 5‐FU, 5‐fluorouracil; phosphor, phosphorylated. and CDH1 KO HCT116 cells with similar IC50 values, but no differences between cell types against 5‐FU (P=0.065), erlo‐ tinib (P=0.516), and saracatinib (P=0.802).

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Activation Assay

    Figure 5. Effects of PD98059 on E‑cadherin and spheroidogeneis of WT and CDH1 KO HCT116 cells. (A) PD98059 decreased cell viability in dose‑dependent manner with a significant difference between WT and CDH1 KO HCT116 cells while ERK activation was similar. (B) PD98059 (20 µM) inhibited ERK activation in both cells, but increased E‑cadherin expression. (C) PD98059 decreased sizes of spheroids in both cell lines. (D) PD98059 significantly increased the expression of β‑catenin and pan‑RAS without affecting the expression of EGFR. Scale bar=200 µm. *P<0.05, **P<0.01, ***P<0.001 vs. vehicle; #P<0.05, ###P<0.001 vs. WT. WT, wild‑type; KO, knock‑out.

    Journal: Oncology Letters

    Article Title: The feasible role of soluble E‑cadherin in spheroidogenesis of HCT116 colorectal cancer cells, a candidate biomarker for liquid biopsy

    doi: 10.3892/ol.2025.14991

    Figure Lengend Snippet: Figure 5. Effects of PD98059 on E‑cadherin and spheroidogeneis of WT and CDH1 KO HCT116 cells. (A) PD98059 decreased cell viability in dose‑dependent manner with a significant difference between WT and CDH1 KO HCT116 cells while ERK activation was similar. (B) PD98059 (20 µM) inhibited ERK activation in both cells, but increased E‑cadherin expression. (C) PD98059 decreased sizes of spheroids in both cell lines. (D) PD98059 significantly increased the expression of β‑catenin and pan‑RAS without affecting the expression of EGFR. Scale bar=200 µm. *P<0.05, **P<0.01, ***P<0.001 vs. vehicle; #P<0.05, ###P<0.001 vs. WT. WT, wild‑type; KO, knock‑out.

    Article Snippet: A, Reagents Name (cat. no.) Company Purpose/action MTT (M6494) Thermo Fisher Scientific Cell viability 5‐FU (F6627); erlotinib (S2156) Sigma‐Aldrich (Merck KGaA) Anti‐cancer drugs Saracatinib (11497) Cayman Chemical T‐PERTM protein extraction reagent (78510) Thermo Fisher Scientific Preparation for western blotting Protease inhibitor cocktail (ab201111); Abcam Preparation for western blotting phosphatase inhibitor cocktail I (ab201112); phosphatase inhibitor cocktail II (ab201113) PierceTM BCA protein assay kit (23227) Thermo Fisher Scientific Preparation for western blotting PD98059 (9900) Cell Signaling Tech MEK‐ERK inhibitor Hematoxylin (H‐3404) Vector Laboratories Counter‐staining Human E‐cadherin protein (ab235682) Abcam Recombinant E‐cadherin protein for spheroidogenesis DECMA‐1 (ab11512) Abcam Anti‐E‐cadherin antibody (intercellular junction marker) for spheroidogenesis Human E‐cadherin SimpleStep ELISA Kit (ab233611) Abcam Soluble E‐cadherin detection B, Antibodies Name (cat. no.) Company Purpose/action Phosphor‐ERK (4370) Cell Signaling Tech Primary antibody EGFR (ab52894) Abcam Primary antibody ERK (sc‐93); β‐catenin (sc‐7199); Santa Cruz Biotechnology Primary antibody pan‐RAS (sc‐166691); β‐actin (sc‐47778); GAPDH (sc‐47724) Anti‐mouse IgG (PI‐2000); anti‐rabbit IgG (PI‐1000) Vector Laboratories Secondary antibody ImmPRESS Reagent kit (MP‐7401) Vector Laboratories Immunocytochemistry 5‐FU, 5‐fluorouracil; phosphor, phosphorylated. and CDH1 KO HCT116 cells with similar IC50 values, but no differences between cell types against 5‐FU (P=0.065), erlo‐ tinib (P=0.516), and saracatinib (P=0.802).

    Techniques: Activation Assay, Expressing

    Surface Expression of Various Growth Factor Receptors in Human Brain Cancer Cell Line (Determined by Flow Cytometry)

    Journal: World Journal of Oncology

    Article Title: Synergistic Effects of Neratinib in Combination With Palbociclib or Miransertib in Brain Cancer Cells

    doi: 10.14740/wjon1873

    Figure Lengend Snippet: Surface Expression of Various Growth Factor Receptors in Human Brain Cancer Cell Line (Determined by Flow Cytometry)

    Article Snippet: Other antibodies for the Western blot analysis, such as the rabbit anti-phospho-EGFR (Tyr1068), HER2 (2242s), phosphor-HER2 (Tyr1221/1222) (2243), phosphor-HER3 (Tyr1289) (4791), phosphor-HER4 (Tyr1284)/EGFR (Tyr1173) (4757), phosphor-MAPK (Tyr202/Tyr204) (4370), phospho-Akt (S473) (4060), phospho-STAT3 (Y705) (9145), phospho-SRC (Y416) (Tyr416) (6942) and β-actin (4970), were all obtained from Cell Signaling Technology Inc. (Hitchin, UK).

    Techniques: Expressing, Cytometry, Fluorescence

    IC 50 Values of Various Agents on HBCCLs as Assessed by SRB Colorimetric Assay: (A) HER-Family Targeting TKIs and Other Downstream Signaling Molecules and (B) Other TKIs and Chemotherapeutic Agents

    Journal: World Journal of Oncology

    Article Title: Synergistic Effects of Neratinib in Combination With Palbociclib or Miransertib in Brain Cancer Cells

    doi: 10.14740/wjon1873

    Figure Lengend Snippet: IC 50 Values of Various Agents on HBCCLs as Assessed by SRB Colorimetric Assay: (A) HER-Family Targeting TKIs and Other Downstream Signaling Molecules and (B) Other TKIs and Chemotherapeutic Agents

    Article Snippet: Other antibodies for the Western blot analysis, such as the rabbit anti-phospho-EGFR (Tyr1068), HER2 (2242s), phosphor-HER2 (Tyr1221/1222) (2243), phosphor-HER3 (Tyr1289) (4791), phosphor-HER4 (Tyr1284)/EGFR (Tyr1173) (4757), phosphor-MAPK (Tyr202/Tyr204) (4370), phospho-Akt (S473) (4060), phospho-STAT3 (Y705) (9145), phospho-SRC (Y416) (Tyr416) (6942) and β-actin (4970), were all obtained from Cell Signaling Technology Inc. (Hitchin, UK).

    Techniques: Colorimetric Assay, Polymer